A Field?Independent Method for the Rapid Generation of Hyperpolarized [1? ¹³ C]Pyruvate in Clean Water Solutions for Biomedical Applications

Hyperpolarization methods in magnetic resonance enhance the signals by several orders of magnitude, opening new windows for real-time investigations dynamic processes vitro and vivo. Here, we propose a field-independent para-hydrogen-based pulsed method to produce rapidly hyperpolarized 13C-labeled substrates. We demonstrate polarizing carboxylic carbon pyruvate moiety purposely designed precursor 24 % at ?22 mT. Following fast purification procedure, measure 8 polarization on free [1-13C]pyruvate clean water solutions physiological conditions 7 T. The enhanced allow monitoring pyruvate-lactate conversion cancer cells, demonstrating potential biomedical applications combination with existing or developing technologies.